Preparation method of high-activity sugarcane anthocyanin

一种高活性甘蔗花色苷的制备方法

Abstract

本发明公开了一种高活性甘蔗花色苷的制备方法。该方法包括:1)取榨汁后的甘蔗渣,晾干,破碎,加入蒸馏水;将纤维素酶,半纤维素酶,果胶酶混合配制复合酶,灭菌,加入到反应液中,浸提,灭酶,离心,真空浓缩,得甘蔗花色苷粗提液;2)将粗提液经装有大孔吸附树脂第一段色谱柱进行除盐,提取剂洗脱后,真空浓缩,得花色苷溶液;3)用酸调节后,再经装有葡聚糖凝胶树脂的第二段色谱柱,收集洗脱液,真空浓缩,浓缩液为花色苷纯化液;4)冷冻干燥,得花色苷纯化物。本发明以甘蔗皮或甘蔗渣为原料提取花色苷,将制糖生产后的废弃物变废为宝,提高了甘蔗的利用率,延长了甘蔗的生产链,大大提高了甘蔗的附加价值。
The invention discloses a preparation method of high-activity sugarcane anthocyanin. The preparation method comprises the following steps: (1) extracting squeezed bagasse, airing, crushing, adding distilled water, mixing cellulase, hemicellulase and pectinase to prepare compound enzyme, carrying out sterilization, adding the compound enzyme into reaction liquid, and carrying out leaching, enzyme deactivation, centrifugation and vacuum concentration, so as to obtain a sugarcane anthocyanin crude extracting solution; (2) desalting the crude extracting solution by virtue of a first-section chromatographic column filled with macroporous adsorption resin, carrying out elution by virtue of an extracting agent, and carrying out vacuum concentration, so as to obtain an anthocyanin solution; (3) adjusting the anthocyanin solution with acid, processing the anthocyanin solution by virtue of a second-section chromatographic column filled with dextrangel resin, collecting eluant, and carrying out vacuum concentration, so as to obtain concentrated liquid, namely anthocyanin purified liquid; and (4) carrying out freeze drying, so as to obtain purified anthocyanin. According to the preparation method, anthocyanin is extracted from the raw material of sugarcane skin or bagasse, and the waste obtained after sugar production is turned into wealth, so that the utilization rate of sugarcanes is increased, the production chain of the sugarcanes is prolonged, and the added value of the sugarcanes is greatly increased.

Claims

Description

Topics

Download Full PDF Version (Non-Commercial Use)

Patent Citations (0)

    Publication numberPublication dateAssigneeTitle

NO-Patent Citations (0)

    Title

Cited By (0)

    Publication numberPublication dateAssigneeTitle